Marine life is a vast resource, providing food, medicine, and raw materials, in addition to helping to support recreation and tourism all over the world. Marine biology is the scientific study of marine life and organisms in the sea, and it has been one aspect of environmental study that helps us to know more about these aquatic life. There are several various marine ecosystems including salt marshes, intertidal zones and cold seeps. Within each of these ecosystems, there are various aquatic life. The aquatic life distributions is affected by any factors including several environmental factors. Over the years, there has been a lot of research on deep marine biosphere systems. And several advances have been made that elevate the quality of research in deep marine biosphere in the present. The various advances include methods in sampling, biomass extraction and quantification, DNA and RNA extraction and various advanced on site mechanical systems. This report discusses the various marine ecosystems in detail, and provides in depth information about the various advances made over the years towards the quality of research in deep marine biosphere.
Table of Contents
1 INTRODUCTION.. 3
2 MARINE ECOSYSTEMS. 4
2.1 TYPES OF MARINE ECOSYSTEMS. 4
2.1.1 SALT MARSHES. 4
2.1.2 INTERTIDAL ZONES. 6
2.1.3 COLD SEEPS. 7
2.2 FACTORS AFFECTING MARINE BIOSPHERE.. 8
2.3 “ENVIRONMENTAL FACTORS THAT AFFECT THE DISTRIBUTION OF MARINE ORGANISMS” 8
3 “RECENT ADVANCES IN MARINE DEEP BIOSPHERE RESEARCH”. 10
3.1 “ADVANCES IN SAMPLE COLLECTION”. 10
3.2 “ADVANCES IN BIOMASS QUANTIFICATION”. 11
3.3 “ADVANCES IN DNA AND RNA EXTRACTION, AMPLIFICATION, AND SEQUENCING”. 12
3.4 “ADVANCES IN ACTIVITY MEASUREMENTS”. 12
3.5 ADVANCES IN UNDERSTANDING ENERGY SUPPLY AND DEMAND.. 12
4 CONCLUSION.. 14
Table of Figures
Figure 1 – Salt Marshes. 5
Figure 2 – Intertidal zone marks displayed on rocks. 6
Figure 3 – A picture showing cold seeps on the left, and Tubeworms on the right. 8
Figure 4 – Newly developed seabed rock drills by the UK.. 10
Figure 5 – The newly developed in-situ electrochemical analyzer. 11
“A large proportion of all life on Earth lives in the ocean.” The exact size of this large proportion is unknown, since many ocean species are still to be discovered. The ocean is a complex three-dimensional world (Marine, 2013) covering approximately 71% of the Earth’s surface. “Marine life is a vast resource, providing food, medicine, and raw materials, in addition to helping to support recreation and tourism all over the world.” At a fundamental level, marine life helps determine the very nature of our planet. Marine organisms contribute significantly to the oxygen cycle, and are involved in the regulation of the Earth’s climate (Foley, et.al., 1991). Shorelines are in part shaped and protected by marine life, and some marine organisms even help create new land (Sousa, 1985).Hence the need to study marine biology.
Marine biology is the scientific study of marine life, organisms in the sea. Given that in biology many phyla, families and genera have some species that live in the sea and others that live on land, marine biology classifies species based on the environment rather than on taxonomy. The habitats studied in marine biology include everything from the tiny layers of surface water in which organisms and abiotic items may be trapped in surface tension between the ocean and atmosphere, to the depths of the oceanic trenches, sometimes 10,000 meters or more beneath the surface of the ocean. Even though so many microorganisms reside in the deep biosphere — about 3 × 1029 cells according to the most recent census (Kallmeyer,et.al., 2012) the energy flux available from buried organic carbon is less than 1% of the photosynthetically fixed carbon on the surface of our planet. (Whitman, et.al., 1998)
Microscopic life undersea is unfathomably differing and still ineffectively caught on. For instance, the part of infections in marine biological communities is scarcely being investigated even in the start of the 21st century. (Suttle, 2005). As tenants of the biggest condition on Earth, microbial marine frameworks drive changes in each worldwide framework. Organisms are in charge of for all intents and purposes all the photosynthesis that happens in the sea, and in addition the cycling of carbon, nitrogen, phosphorus and different supplements and follow components (Georgia, 2015).
The revelation of microbial life underneath the surface of the mainlands and the seabed has demonstrated that a substantial extent of the considerable number of microscopic organisms and archaea on Earth live in the profound biosphere (Whitman, et.al., 1998) and that the vitality supply for microbial life that is available in this condition traverses many requests of greatness.
2 MARINE ECOSYSTEMS
Marine biological systems are fundamental for the general wellbeing of both marine and earthly situations. As indicated by the World Resource Center, seaside territories represent around 33% of sea life organic profitability. Estuarine environments, for example, salt swamps, seagrass knolls and mangrove backwoods, are among the most profitable biological communities on the planet. Coral reefs give sustenance and safe house to the most elevated amounts of marine assorted variety on the planet (EPA, 2007).
Marine biological systems are amongst the biggest of Earth’s aquatic environments. Illustrations incorporate salt swamps, intertidal zones, estuaries, tidal ponds, mangroves, coral reefs, the remote ocean, and the ocean bottom. They can be appeared differently in relation to freshwater biological systems, which have a lower salt substance. Marine waters cover 66% of the surface of the Earth. Such places are considered environments on the grounds that the vegetation underpins the creature life and the other way around (Marine, 2007).
Marine biological systems more often than not have an extensive biodiversity and are thusly thought to have a decent protection against intrusive species. Nonetheless, exemptions have been watched, and the components mindful in deciding the accomplishment of an attack are not yet clear.
2.1 TYPES OF MARINE ECOSYSTEMS
2.1.1 SALT MARSHES.
A salt marsh or saltmarsh, additionally acknowledged as much a worried powder pore and a tidal marsh, is an impartial ecosystem within the top unbiased intertidal area within region or originate salt water yet brackish lotos, so is usually flooded through the tides. It is ruled by using close stands over salt-tolerant vegetation for example herbs, grasses, then vile shrubs (Adam, 1990; Allen, et.al., 1992).
Most salt swamps have a low geography with low rises yet an immense wide territory, making them colossally famous for human populaces. Salt swamps are situated among various landforms in view of their physical and geomorphological settings. Such bog landforms incorporate deltaic bogs, estuarine, back-obstruction, open drift, embayment and suffocated valley swamps. Deltaic swamps are related to expansive waterways where many happen in Southern Europe, for example, the Camargue, France in the Rhone delta or the Ebro delta in Spain. They are additionally broad inside the waterways of the Mississippi Delta in the United States (Allen,et.al., 1992).
The low physical vitality and high grasses give an asylum to creatures. Numerous marine fish utilize salt swamps as nursery reason for their young before they move to vast waters. Winged creatures may raise their young among the high grasses, in light of the fact that the bog gives both asylum from predators and inexhaustible nourishment sources which incorporate fish caught in pools, bugs, shellfish, and worms. (Scott, et.al. 2014)
Salt bogs are now and again incorporated into tidal ponds, and the distinction isn’t exceptionally denoted; the Venetian Lagoon in Italy, for instance, is comprised of these sorts of creatures and additionally living beings having a place with this biological community. They bigly affect the biodiversity of the region. Salt swamp environment includes complex nourishment networks which incorporate essential makers (vascular plants, macroalgae, diatoms, epiphytes, and phytoplankton), essential buyers (zooplankton, macrozoa, molluscs, bugs), and optional purchasers (Vernberg, 1993).
Figure 1 – Salt Marshes
2.1.2 INTERTIDAL ZONES
The intertidal zone, otherwise called the foreshore and seashore and at times alluded to as the littoral zone, is the territory that is above water at low tide and submerged at high tide (as it were, the zone between tide marks). This territory can incorporate various kinds of living spaces, with many sorts of creatures, for example, starfish, ocean urchins, and various types of coral. The outstanding zone likewise incorporates soak rough bluffs, sandy shorelines, or wetlands (e.g., huge mudflats). The intertidal zone is likewise home to numerous few species from various taxa including Porifera, Annelids, Coelenterates, Mollusks, shellfish, Arthropods, etc.(Walag, et.al., 2016).
The area contains a high assorted variety of species, and the zonation made by the tides causes species extents to be packed into extremely limit groups. This makes it moderately easy to think about species over their whole cross-shore go, something that can be to a great degree troublesome in, for example, earthbound natural surroundings that can extend a huge number of kilometers. Groups on wave-cleared shores likewise have high turnover because of unsettling influence, so it is conceivable to watch environmental progression over years as opposed to decades. The tunneling spineless creatures that make up huge bits of sandy shoreline biological systems are known to movement moderately awesome separations in cross-shore bearings as shorelines change on the request of days, semilunar cycles, seasons, or years (Dugan, 2013).
2.1.3 COLD SEEPS
Cold seeps are seafloor ecosystems fueled by chemical energy originating from microbial trans- formation of hydrocarbons and sulfide. They are characterized by the largest biomass and highest productivity of all deep-sea ecosystems (Jørgensen and Boetius, 2007; Levin and Sibuet 2012; Boetius and Wenzho ? fer 2013)
Organic research in colds seeps and aqueous vents has been for the most part centered around the microbiology and the noticeable large scale spineless creatures flourishing with chemosynthetic microorganisms (Vanreusel, et.al. 2010). The main sort of living creatures to exploit this remote ocean vitality source is bacteria (Hsing, 2010).Aggregating into bacterial mats at chilly leaks, these microbes process methane and hydrogen sulfide (another gas that rises up out of leaks) for vitality. (Hsing, 2010).
Amid this underlying stage, when methane is moderately plenteous, thick mussel beds likewise shape close to the cool leak. For the most part made out of species in the family Bathymodiolus, these mussels don’t straightforwardly devour sustenance .Instead, they are sustained by advantageous microbes that additionally create vitality from methane, like their relatives that frame mats. (Hsing, 2010).
Amid a period enduring up to quite a few years, these stone arrangements pull in siboglinid tubeworms, which settle and develop alongside the mussels. Like the mussels, tubeworms depend on chemosynthetic microorganisms (for this situation, a sort that requirements hydrogen sulfide rather than methane) for survival. Consistent with any advantageous relationship, a tubeworm likewise accommodates their bacteria by appropriating hydrogen sulfide from the earth. The sulfide originates from the water, as well as mined from the dregs through a broad “root” framework a tubeworm “shrub” sets up in the hard, carbonate substrate. A tubeworm hedge can contain many individual worms, which can grow a meter or more over the residue. (Hsing, 2010).
2.2 FACTORS AFFECTING MARINE BIOSPHERE
1. Biotic factors- all living organisms inhabiting the marine (ex: bacteria, etc.)
2. Abiotic factors- nonliving (physical) parts of the environment (ex: temperature, soil, light, moisture, air currents.
2.3 “ENVIRONMENTAL FACTORS THAT AFFECT THE DISTRIBUTION OF MARINE ORGANISMS”
· Sunlight assumes a basic part in the marine condition. Photosynthetic creatures are the base of almost every nourishment web in the sea and are reliant upon daylight to give vitality to deliver natural atoms. Light is additionally vital for vision, as many number of life forms depend on this to catch prey, maintain a strategic distance from predation, and impart, and for species recognition in reproduction.
· Temperature; the temperature of shallow subtidal and intertidal territories might be always showing signs of change, and living beings living in these conditions should have the capacity to adjust to these progressions. On the other hand, in the open seas and remote oceans, the temperatures may remain generally steady, so living beings don’t should be as versatile.
· Saltiness is the measure of the grouping of broken up natural salts in the water segment and is estimated in parts per thousand (‰). Life forms must keep up a legitimate adjustments of water and salts inside their tissues.
· Pressure: At sea height, the pressure exerted is 1 atm. Water is considerably denser than air, and for each 10 meters plummet beneath ocean level, the weight increments is by 1 atm. Life forms found in the profound seas expect adjustments to enable them to get by at extraordinary pressures.
· Metabolic Requirements: Living beings require an assortment of natural and inorganic materials to process, develop, and recreate. The substance content of saltwater gives a few of the supplements required by marine life forms. Nitrogen and phosphorous are required by all photosynthesizing plants or plant-like living organisms in the sea.
3 “RECENT ADVANCES IN MARINE DEEP BIOSPHERE RESEARCH”
3.1 “ADVANCES IN SAMPLE COLLECTION”
Latest discoveries of spatial and fleeting dispersion and rates of microbial action in the marine profound biosphere have been made conceivable by propels in test gathering and information investigation. Albeit logical sea penetrating has proceeded since the late 1960s, and routinely gathers gauge geophysical and geochemical parameters from cored material, development and application (Expedition 327 Scientists, 2011; Expedition 329 Scientists, 2011a). Some new advances are as follows;
• A newly developed coring devices for deeper cores that are 10m and more (Røy et al., 2012).
• Newly developed seabed rock drills by the UK (Petersen et al., 2007) and Germany (Freudenthal and Wefer, 2009; Krastel et al., 2011) propagates the collection of in-situ rock materials and deposits of hydrothermal settings.
• It is now possible to collect high quality fluid samples from the sea floor due to the new Installation of a refurbished and advanced Circulation Obviation Retrofit Kits (CORKs). (Wheat et al. (2011)
• Ships are now stocked with modern freezers for the purpose of storing samples for DNA and RNA purposes.
• An electrochemical analyzer that analyses data on site (e.g., Edwards et al., 2011a).
In order to collect pristine fluids and sediments from hydrothermal vents, plumes, and microbial mats several on site fluid pumping systems (Breier et al., 2012).
3.2 “ADVANCES IN BIOMASS QUANTIFICATION”
• Deep sediments have been projected to have contained 3.55×1030 total microbial cells based on the densities of the accompanying cells. They are determined by a technique known as the epi?uorescence microscopy. The carbon- rich sediment is collected close to shore and tested (Cragg et al., 1990; Parkes et al., 1994; Whitman et al., 1998).
• Re-visiting the earlier calculations and with the several initial cell counts from low biomass sites, it has been estimated that the number of cells reduces by an order of magnitude, from 3.55×1030 to 2.9×1029 cells. This is only with respect to sub-seafloor sediments (Kallmeyer et al., 2012)
• Microbial productivity in basalt systems (as their primary productivity) based on an advancement perspective is calculated to be 0.5Pg carbon, or in order units 2×1024 cells in carbon worth. (Bach and Edwards, 2003).
• A few advances in test handling have now as of late empowered cell identification in even lower biomass tests. These incorporate advancements in cell division from the dregs framework to think (Kallmeyer et al., 2008) biomass and additionally computerization of cell tallying (Morono et al., 2009).
3.3 “ADVANCES IN DNA AND RNA EXTRACTION, AMPLIFICATION, AND SEQUENCING”
• Perfection in the extraction of DNA and RNA. (Mills et al., 2008, 2012a)
• Single cell based and “omic” techniques are poised to yield important new insights into the functioning of microorganisms in these habitats. (Stepanauskas and Sieracki, 2007; Stepanauskas, 2012)
• There has been an exploration that has identified a huge mass of the sediment Archaea that was presumed to be almost heterotrophic. (Lloyd et al., 2013)
• Several subsurface organisms develop several adaptation skills to adapt to the diverse energy deficiency within the subsurface. This has also been studied.
• Through several comparative analysis study on eukaryotes, microbiologist have identified differences between eukaryotes with active fungi fractions and those without. (Orsi et al., 2013a).
3.4 “ADVANCES IN ACTIVITY MEASUREMENTS”
• Some microbial action estimations can be specifically quanti?ed utilizing steady and radio-isotope tracer-based methods.
• Some activity measurements done are :
o Measuring the mechanism with respect to time for which sulfate-reducing bacteria react with and destroy sulfate in seafloor sediment. (Jørgensen, 1982; Kallmeyer and Boetius, 2004; Jørgensen et al., 2006)
o Measuring the hydrogenase activity in marine sediments using tritiated hydrogen (moles per area per time). (Nunoura et al., 2009; Soffientino et al., 2009)
o User-friendly analytical models between converting chemical gradients of undertaking determinations. (Berg et al., 1996; Aguillera et al., 2005; Thullner et al., 2007; LaRowe et al., 2008; Regnier et al., 2011).
3.5 ADVANCES IN UNDERSTANDING ENERGY SUPPLY AND DEMAND
• Thermodynamic models have been utilized to evaluate the vitality free market activity in different biological communities since vitality accessibility is one of the key factors that influences microbial movement level (Van Briesen, 2002).
• Energy for active microorganisms from the catalysis of redox reactions can be estimated using the Gibbs energies of potential catabolic reactions.
• Catabolic reactions release some form of energy as by product and this energy determines the growing potential of several microorganisms, and as such, the rate and quantity of biomass produced in a given setting depends on it too. (Jørgensen, 2011).
Quantifying the charges at which microbial recreation into the subsurface occurs is a difficult endeavor, yet flourishing a grasp over it charges is very crucial to decide the impact regarding subsurface lifestyles of Earth’s global biogeochemical cycles, and for grasping how microorganisms in this “extreme” environments survive.
Information based on the biodiversity patterns of very deep-sea organisms varying spatially and temporally, is important to estimate the responses of deep-sea ecosystems to current and future environmental and anthropogenic changes.